Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/7012
Title: Novel smoothened antagonists as anti-neoplastic agents for the treatment of osteosarcoma
Authors: Bernardini, Giulia 
Geminiani, Michela 
Gambassi, Silvia
Orlandini, Maurizio 
Petricci, Elena 
Marzocchi, Barbara 
Laschi, Marcella
Taddei, Maurizio 
Manetti, Fabrizio 
Santucci, Annalisa 
Keywords: apoptosis, cancer, CyQuant, GLI-1, hedgehog pathway, osteosarcoma, research resource identifiers—RRID, SMO inhibitors
Issue Date: 2018
Project: None 
Journal: JOURNAL OF CELLULAR PHYSIOLOGY
Abstract: 
Osteosarcoma (OS) is an ultra-rare highly malignant tumor of the skeletal system affecting mainly children and young adults and it is characterized by an extremely aggressive clinical course. OS patients are currently treated with chemotherapy and complete surgical resection of cancer tissue. However, resistance to chemotherapy and the recurrence of disease, as pulmonary metastasis, remain the two greatest challenges in the management, and treatment of this tumor. For these reasons, it is of primary interest to find alternative therapeutic strategies for OS. Dysregulated Hedgehog signalling is involved in the development of various types of cancers including OS. It has also been implicated in tumor/stromal interaction and cancer stem cell biology, and therefore presents a novel therapeutic strategy for cancer treatment. In our work, we tested the activity of five potent Smoothened (SMO) inhibitors, four acylguanidine and one acylthiourea derivatives, against an OS cell line. We found that almost all our compounds were able to inhibit OS cells proliferation and to reduce Gli1 protein levels. Our results also indicated that SMO inhibition in OS cells by such compounds, induces apoptosis with a nanomolar potency. These findings suggest that inactivation of SMO may be a useful approach to the treatment of patients with OS.
Description: 
130893
URI: http://hdl.handle.net/20.500.12779/7012
ISSN: 0021-9541
DOI: 10.1002/jcp.26330
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