Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/6553
Title: EPR and LC-MS studies on the mechanism of industrial dye decolorization by versatile peroxidase from Bjerkandera adusta
Authors: Baratto, Maria Camilla 
Karla Juarez-Moreno
Pogni, Rebecca 
Basosi, Riccardo 
Rafael Vazquez-Duhalt
Keywords: Azo dyes; Bjerkandera adusta; Enzymatic decolorization; Phthalocyanines dyes; Versatile peroxidase
Issue Date: 2015
Project: None 
Journal: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH INTERNATIONAL
Abstract: 
The mechanisms of industrial dye transformation by versatile peroxidase were elucidated. Purified versatile peroxidase from Bjerkandera adusta was able to decolorize different classes of dyes including azo and phthalocyanines, but unable to transform any of the anthraquinones tested. Kinetic constants for selected dyes were determined and the transformation products were analyzed by EPR spectroscopy and mass spectrometry. The EPR and MS analyses of the enzymatic decolorization products showed the cleavage of the azo bond in azo dyes and the total disruption of the phthalocyaninic ring in phthalocyanine dyes. The EPR analysis on two coppercontaining dyes, reactive violet 5 (azo) and reactive blue 72 (phthalocyanine), showed that the transformation can or not break the metal-ion coordination bond according the dye nature. The role of the catalytic Trp172 in the dye transformation by a long-range electron transfer pathway was confirmed and the oxidation mechanisms are proposed and discussed.
Description: 
76451
URI: http://hdl.handle.net/20.500.12779/6553
ISSN: 1614-7499
DOI: 10.1007/s11356-014-4051-9
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