Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/6408
DC FieldValueLanguage
dc.contributor.authorOrlandini, Maurizioen_us
dc.contributor.authorGalvagni, Federicoen_us
dc.contributor.authorBardelli, Men_us
dc.contributor.authorRocchigiani, Marinaen_us
dc.contributor.authorLentucci, Cen_us
dc.contributor.authorAnselmi, Fen_us
dc.contributor.authorZippo, Aen_us
dc.contributor.authorBini, Lucaen_us
dc.contributor.authorOliviero, Salvatoreen_us
dc.date.accessioned2021-03-30T16:01:56Z-
dc.date.available2021-03-30T16:01:56Z-
dc.date.issued2014-
dc.identifier.issn1949-2553en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12779/6408-
dc.description60166en_US
dc.description.abstractThe inhibition of tumor angiogenesis is one of the main challenges in cancer therapy. With the aim of developing monoclonal antibodies able to inhibit angiogenesis, we immunized mice with proliferating human umbilical vein endothelial cells. We generated a library of monoclonal antibodies able to recognize antigens expressed on endothelial cells and screened the antibodies for their ability to inhibit endothelial cell proliferation, migration, and sprouting in vitro. Here, we show that the antibody, designated as 4E1, is able to neutralize the formation of new vessels both in vitro and in vivo without affecting endothelial cell survival. By mass spectrometry we identified CD93 as the antigen bound by 4E1 and mapped the recognized epitope. CD93 is a transmembrane protein heavily glycosylated preferentially expressed in the vascular endothelium. CD93 silencing by lentiviral-mediated small hairpin RNA expression impairs human endothelial cell proliferation, migration, and sprouting. Altogether these findings reveal 4E1 as a novel antiangiogenic antibody and identify CD93 as a new target suitable for antiangiogenic therapy.en_US
dc.language.isoenen_US
dc.relationNoneen_US
dc.relation.ispartofONCOTARGETen_US
dc.titleThe characterization of a novel monoclonal antibody against CD93 unveils a new antiangiogenic target.en_US
dc.typeArticleen_US
dc.identifier.doi10.18632/oncotarget.1887en_US
dc.identifier.pmid24809468en_US
dc.identifier.scopus2-s2.0-84901227833en_US
dc.identifier.isiWOS:000336966600034en_US
dc.identifier.urlhttp://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=1887en_US
dc.relation.volume5en_US
dc.relation.issue9en_US
dc.description.firstpage2750en_US
dc.description.lastpage2760en_US
dc.description.thirdmissionNot applicableen_US
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.orcid0000-0002-6112-4889-
crisitem.author.orcid0000-0003-1967-9554-
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