Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/6408
Title: The characterization of a novel monoclonal antibody against CD93 unveils a new antiangiogenic target.
Authors: Orlandini, Maurizio 
Galvagni, Federico 
Bardelli, M
Rocchigiani, Marina
Lentucci, C
Anselmi, F
Zippo, A
Bini, Luca
Oliviero, Salvatore 
Issue Date: 2014
Project: None 
Journal: ONCOTARGET
Abstract: 
The inhibition of tumor angiogenesis is one of the main challenges in cancer therapy. With the aim of developing monoclonal antibodies able to inhibit angiogenesis, we immunized mice with proliferating human umbilical vein endothelial cells. We generated a library of monoclonal antibodies able to recognize antigens expressed on endothelial cells and screened the antibodies for their ability to inhibit endothelial cell proliferation, migration, and sprouting in vitro. Here, we show that the antibody, designated as 4E1, is able to neutralize the formation of new vessels both in vitro and in vivo without affecting endothelial cell survival. By mass spectrometry we identified CD93 as the antigen bound by 4E1 and mapped the recognized epitope. CD93 is a transmembrane protein heavily glycosylated preferentially expressed in the vascular endothelium. CD93 silencing by lentiviral-mediated small hairpin RNA expression impairs human endothelial cell proliferation, migration, and sprouting. Altogether these findings reveal 4E1 as a novel antiangiogenic antibody and identify CD93 as a new target suitable for antiangiogenic therapy.
Description: 
60166
URI: http://hdl.handle.net/20.500.12779/6408
ISSN: 1949-2553
DOI: 10.18632/oncotarget.1887
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