Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/6378
Title: Nylon 6 film and nanofiber carriers: preparation and laccase immobilization performance
Authors: Fatarella, Enrico
Spinelli, Daniele
Ruzzante, Marco
Pogni, Rebecca 
Keywords: Nylon carriers Fungal laccase Immobilized enzyme
Issue Date: 2014
Project: None 
Journal: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Abstract: 
Enzyme immobilization has attracted continuous attention in the field of fine chemistry, biomedicine and biosensor. Polyamides are promising materials to promote immobilization: thus, nylon 6 film and nanofiber carriers(prepared by electrospinning method) have been investigated. The enzyme (i.e.lac- case from Trametes versicolor) was covalently immobilized onto spacer-arm attached carriers after acidic hydrolysis. The amount of immobilized enzyme on the nylon film and nanofibers was 59.4% and 71.0% respectively. The maximum activity (Vmax) and Michaelis–Menten constant (Km) of laccase immobilized on functionalized carriers were determined.The operationa and thermal stabilities of the immobilized laccase were improved compared to free counter part. The use of nylon carriers for enzyme immobi- lization has shown interesting properties to be used as biocatalytic material in industrial applications. Furthermore, nylon carriers are cheap and could be produced at large scale.
Description: 
76414
URI: http://hdl.handle.net/20.500.12779/6378
ISSN: 1381-1177
DOI: 10.1016/j.molcatb.2014.01.012
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