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Title: Cigarette smoke induces alterations in the drug-binding properties of human serum albumin
Authors: Clerici, Marco
Colombo, Graziano
Secundo, Francesco
Gagliano, Nicoletta
Colombo, Roberto
Portinaro, Nicola
Giustarini, Daniela 
Milzani, Aldo
Rossi, Ranieri 
Dalle Donne, Isabella
Keywords: Cigarette smoke; Human serum albumin; Naproxen; Oxidation; Protein conformation; Salicylic acid; Curcumin; Genistein; Humans; Hydrophobic and Hydrophilic Interactions; Kinetics; Naproxen; Oxidation-Reduction; Protein Binding; Protein Conformation; Proteolysis; Salicylic Acid; Serum Albumin; Smoke; Smoking; Ligands; Molecular Biology; Molecular Medicine; Hematology; Cell Biology
Issue Date: 2014
Project: None 
Albumin is the most abundant plasma protein and serves as a transport and depot protein for numerous endogenous and exogenous compounds. Earlier we had shown that cigarette smoke induces carbonylation of human serum albumin (HSA) and alters its redox state. Here, the effect of whole-phase cigarette smoke on HSA ligand-binding properties was evaluated by equilibrium dialysis and size-exclusion HPLC or tryptophan fluorescence. The binding of salicylic acid and naproxen to cigarette smoke-oxidized HSA resulted to be impaired, unlike that of curcumin and genistein, chosen as representative ligands. Binding of the hydrophobic fluorescent probe 4,4'-bis(1-anilino-8-naphtalenesulfonic acid) (bis-ANS), intrinsic typtophan fluorescence, and susceptibility to enzymatic proteolysis revealed slight changes in albumin conformation. These findings suggest that cigarette smoke-induced modifications of HSA may affect the binding, transport and bioavailability of specific ligands in smokers.
ISSN: 1079-9796
DOI: 10.1016/j.bcmd.2013.12.001
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