Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/6286
Title: FOSL1 Controls the Assembly of Endothelial Cells into Capillary Tubes by Direct Repression of  alphav and  beta3 Integrin Transcription
Authors: S., Evellin
Galvagni, Federico 
A., Zippo
F., Neri
Orlandini, Maurizio 
D., Incarnato
D., Dettori
S., Neubauer
H., Kessler
E. F., Wagner
S., Oliviero
Keywords: angiogenesis; transcription factor; FOSL1; FRA-1; HUVEC; INTEGRINS
Issue Date: 2013
Project: None 
Journal: MOLECULAR AND CELLULAR BIOLOGY
Abstract: 
To form three-dimensional capillary tubes, endothelial cells must establish contacts with the extracellular matrix that provides signals for their proliferation, migration, and differentiation. The transcription factor Fosl1 plays a key role in the vasculogenic and angiogenic processes as Fosl1 knockout embryos die with vascular defects in extraembryonic tissues. Here, we show that Fosl1−/− embryonic stem cells differentiate into endothelial cells but fail to correctly assemble into primitive capillaries and to form tube-like structures. FOSL1 silencing affects in vitro angiogenesis, increases cell adhesion, and decreases cell mobility of primary human endothelial cells (HUVEC). We further show that FOSL1 is a repressor of αv and β3 integrin expression and that the down-modulation of αvβ3 rescues the angiogenic phenotype in FOSL1-silenced HUVEC, while the ectopic expression of αvβ3 alone reproduces the phenotypic alterations induced by FOSL1 knockdown. FOSL1 represses the transcription of both αv and β3 integrin genes by binding together with JunD to their proximal promoter via the transcription factor SP1. These data suggest that FOSL1-dependent negative regulation of αvβ3 expression on endothelial cells is required for endothelial assembly into vessel structures.
Description: 
58139
URI: http://hdl.handle.net/20.500.12779/6286
ISSN: 0270-7306
DOI: 10.1128/MCB.01054-12
Appears in Collections:Publications

Show full item record

Page view(s)

1
Last Week
0
Last month
1
checked on May 5, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.