Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/6259
Title: A spectroscopic characterization of a phenolic natural mediator in the laccase biocatalytic reaction
Authors: Martorana, Andrea
Sorace, A.
Boer, H.
Vazquez Duhalt, R.
Basosi, Riccardo 
Baratto, Maria Camilla 
Keywords: Acetosyringone; Radical intermediate; Laccase; Multifrequency ESR; NALDI-TOF MS
Issue Date: 2013
Project: None 
Journal: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Abstract: 
Multi-frequency ESR combined with NALDI-TOF MS has been used for the characterization of 3,5-dimethoxy-4-hydroxyacetophenone radical intermediate and y-products formed during the Coriolopsis gallica laccase catalytic reaction. A stable radical species is formed and an intense and well-structured ESR spectrum was detected and fully characterized at S-, X- and W-bands. The presence of by-products generated as the result of by-reactions has been investigated and analyzed through NALDI-TOF MS, per-forming the experiments versus time. The superior radical stability of such phenoxy radical, due to steric hindrance in ortho to the phenol group and the great delocalization of the unpaired electron on the acetyl substituent, makes acetosyringone particularly interesting for biotechnological applications. This represents a good example for the development of new stable laccase mediator molecules.
Description: 
58569
URI: http://hdl.handle.net/20.500.12779/6259
ISSN: 1381-1177
DOI: 10.1016/j.molcatb.2013.08.013
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