Please use this identifier to cite or link to this item:
|Title:||Immobilization of fungal (Trametes versicolor) laccase onto Amberlite IR-120 H beads: Optimization and characterization||Authors:||Spinelli, Daniele
Angelo Di, Michele
|Keywords:||Fungal laccase; Oxidoreductase; Amberlite||Issue Date:||2013||Project:||None||Journal:||PROCESS BIOCHEMISTRY||Abstract:||
Laccase from Trametes versicolor was immobilized on Amberlite IR-120 H beads. Maximum immobilization obtained was 78.7% at pH = 4.5 and temperature T = 45 ◦C. Kinetic parameters, Km and Vmax values, were determined respectively as 0.051 mM and 2.77 ×10−2 mM/s for free and 4.70 mM and 5.27 × 10−3 mM/s for immobilized laccase. The Amberlite–laccase system showed a 30% residual activity after 7 cycles. On the other hand, the loss of activity for free laccase after 7 days of storage at 4 ◦C was 18.5% in comparison to Amberlite–laccase system with a loss of 1.4%, during the same period. Improved operational, thermal and storage stabilities of the immobilized laccase were obtained compared to the free counterpart. Therefore, the use of low-cost matrices, like Amberlite for enzyme immobilization represents a promising product for enzymatic industrial applications.
|Appears in Collections:||Publications|
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.