Please use this identifier to cite or link to this item:
Title: GC-MS profiling of the phytochemical constituents of the oleoresin from Copaifera langsdorffii Desf. and a preliminary in vivo evaluation of its antipsoriatic effect.
Authors: Fabrizio, Gelmini
Giangacomo, Beretta
Anselmi, Cecilia
Centini, Marisanna 
Paolo, Magni
Assimiliano, Ruscica
Alberto, Cavalchini
Roberto Maffei, Facino
Keywords: Copaifera langsdorffii Desf.; Chemical composition; GC-MS analysis; Diterpenes and diterpene acids; Cytokines secretion; Nf-kB translocation; Psoriasis
Issue Date: 2013
Project: None 
Copaiba is the oleoresin (OR) obtained from Copaifera (Fabaceae), a neotropical tree which grows inAmazon regions. The balsam, constituted by an essential oil and a resinous fraction is used as folkloristicremedy in the treatment of several inflammatory diseases and for its antioxidant and antibacterialproperties. Aim of this work was (a) to carry out a characterization by GC–MS of the volatile and nonvolatileconstituents of Copaifera langsdorffii Desf. oleoresin (OR); (b) to investigate the mechanism ofits anti-inflammatory activity; (c) to evaluate its antipsoriatic effect after oral intake/topical application.The volatile fraction (yield: 22.51%, w/w) shows: -bergamotene (48.38%), -himachalene (11.17%), -selinene (5.00%) and -caryophyllene (5.47%). The OR residue (77.49%, w/w), after derivatization, showedas main constituents the following compounds: copalic, abietic, daniellic, lambertinic, labd-7-en-15-oic,pimaric, isopimaric acids and kaur16-en18-oic acid.Preincubation of LPS-stimulated human THP-1 monocytes with increasing concentrations of the ORpurified fraction (OR-PF), containing diterpene acids, diterpenes and sesquiterpenes, reduced the releaseof pro-inflammatory cytokines (IL-1, IL-6, TNF) in a dose-range of 0.1–10 M.In addition, in cell culture system of human THP-1 monocytes, 1 M OR-PF counteracts LPS-drivenNF-B nuclear translocation.In a preliminary clinical trial three patients affected by chronic psoriasis, treated with oral intake ortopical application of the OR, exhibited a significant improvement of the typical signs of this disease, i.e.erythema, skin thickness, and scaliness.In conclusion, the results of this work, beside an extensive analytical characterization of the OR chemicalcomposition, provide strong evidences that its anti-inflammatory activity is related to the inhibition ofthe NF-B nuclear translocation, and consequently of proinflammatory cytokines secretion.
ISSN: 0378-5173
DOI: 10.1016/j.ijpharm.2012.08.021
Appears in Collections:Publications

Show full item record

Page view(s)

Last Week
Last month
checked on May 8, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.