Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/6235
Title: Quercetin relaxes rat tail main artery partly via a PKG-mediated stimulation of KCa1.1 channels
Authors: Iozzi, Donata
Schubert, R.
Kalenchuk, V. U.
Neri, Annalisa
Sgaragli, GIAN PIETRO
Fusi, Fabio 
Saponara, Simona
Issue Date: 2013
Project: None 
Journal: ACTA PHYSIOLOGICA
Abstract: 
AIM: Protein kinases, activated by vasodilator substances, affect vascular function by regulating large conductance Ca(2+) -activated K(+) (KCa 1.1) channels. Thus, the aim of the present investigation was to address the hypothesis that quercetin-induced vasorelaxation is caused by a PKG-mediated stimulation of KCa 1.1 currents. METHODS: Single freshly isolated myocytes and endothelium-denuded rings of the rat tail main artery were employed for electrophysiological and contractility measurements respectively. RESULTS: Quercetin relaxed vessels and increased KCa 1.1 currents in a concentration-dependent manner: both effects were antagonized by the specific KCa 1.1 channel blocker iberiotoxin. Stimulation of KCa 1.1 currents was fully reversible upon drug washout, markedly reduced by Rp-8-Br-PET-cGMPs, a PKG-inhibitor, but not affected by catalase. Quercetin shifted by 34.3 mV the voltage dependence of KCa 1.1 channel activation towards more negative membrane potentials without affecting its slope. Under conditions of tight functional coupling between sarcoplasmic reticulum Ca(2+) release sites and KCa 1.1 channels, quercetin decreased both the frequency and the amplitude of KCa 1.1 transient currents in a ryanodine-like manner. CONCLUSION: The natural flavonoid quercetin relaxes the rat tail main artery partly via a PKG-mediated stimulation of smooth muscle KC a 1.1 channels.
Description: 
59624
URI: http://hdl.handle.net/20.500.12779/6235
ISSN: 1748-1708
DOI: 10.1111/apha.12083
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