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Title: Effect of quercetin, rutin, naringenin and epicatechin on lipid peroxidation induced in human sperm.
Authors: Moretti, Elena
Mazzi, Lucia
Terzuoli, Gaia
Bonechi, Claudia 
Iacoponi, Francesca
Martini, S
Rossi, Claudio 
Collodel, Giulia
Issue Date: 2012
Project: None 
Quercetin, Rutin, Naringenin, Epicatechin are flavonoids with many properties, including antioxidant power. We evaluated, in vitro, the cytotoxicity of these flavonoids (20μM, 30μM, 50μM, 100μM, 200μM, 400μM) in swim up selected human sperm. The antioxidant activity was tested against tert-butylhydroperoxide induced lipid peroxidation using C11-BODIPY581/591 probe and transmission electron microscopy. A significant dose dependent effect on sperm viability (P<0.001) and motility (P<0.001) was observed. Lipid peroxidation was decreased in samples treated with quercetin and rutin (20-30 μM) vs samples incubated with tert-butylhydroperoxide alone. Naringenin (30-50 μM) showed a low protective effect and epicatechin (100μM) was not efficacious. Transmission electron microscopy analysis confirmed the protective action of rutin and in particular quercetin on damages induced by lipid peroxidation. These results underlined the scavenger properties of quercetin and rutin. A possible role of these compounds in supplementation of media used during semen handling deserves attention and further studies.
ISSN: 0890-6238
DOI: 10.1016/j.reprotox.2012.10.002
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