Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/6097
Title: IN VITRO BIOCOMPATIBILITY OF NEW PVA-BASED HYDROGELS AS VITREOUS BODY SUBSTITUTES
Authors: Lamponi, Stefania 
Leone, Gemma 
M., Consumi
G., Greco
Magnani, Agnese 
Keywords: Hydrogels; cytotoxicity; biocompatibility; vitreous body; fibroblasts; endothelial cells; human lens cells
Issue Date: 2012
Project: None 
Journal: JOURNAL OF BIOMATERIALS SCIENCE POLYMER EDITION
Abstract: 
In order to synthesize injectable hydrogels suitable as vitreous body substitutes, a new method based on the use of trisodium trimetaphosphate (STMP) to cross-link PVA was recently proposed. Hydrogels with differentmolar ratios between STMP and PVA were realised. The aim of the present study was the evaluation of the biocompatibility of the different STMP/PVA hydrogels synthesised by analysing the effects of their in vitro interaction with cultures of mouse fibroblasts NIH3T3, primary human microvascular endothelial cells adult (HMVECad) and human lens cells. Cytotoxicity of hydrogels was first evaluated by analysing cell density and proliferation. Morphological and morphometric analysis of cell in contact with hydrogels was then performed using light microscopy and scanning electron microscopy, respectively. Moreover, cell adhesion and growth onto the hydrogels surface was evaluated and correlated to the amount of adsorbed proteins. At last, the biocompatibility of the sheared STMP/PVA 1:8 hydrogel was tested. The in vitro data of all the STMP/PVA hydrogels demonstrated their good biocompatibility, and indicated that the 1:8 sample was the most promising as vitreous body substitute.
Description: 
27182
URI: http://hdl.handle.net/20.500.12779/6097
ISSN: 0920-5063
DOI: 10.1163/092050611X554499
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