Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5998
Title: N-Linked Peptidoresorc[4]arene-Based Receptors as Noncompetitive Inhibitors for alpha-Chymotrypsin
Authors: D'Acquarica, I
Cerreto, A
Delle Monache, G
Subrizi, F
Boffi, A
Tafi, Andrea 
Forli, S
Botta, B.
Issue Date: 2011
Project: None 
Journal: JOURNAL OF ORGANIC CHEMISTRY
Abstract: 
This paper deals with the design, synthesis, andevaluation of a new series of receptors for protein surfacerecognition. The design of these agents is based around theattachment of four constrained dipeptide chains onto a centralresorc[4]arene scaffold. By varying the sequence, nature, andstereochemistry of the chains we prepared anionically functionalizedN-linked peptidoresorc[4]arenes 12, 13, and 17 by Pd/C-catalyzed hydrogenation of the corresponding benzyl esters10, 11, and 16. From this family of receptors we have identifiednoncompetitive inhibitors of R-chymotrypsin (ChT), whichfunction by binding to the surface of the enzyme in theneighborhood of the active site cleft (Ki values ranging from12.4 ( 5.1 μM for free carboxylic acid (þ)-12b to 0.76 (0.14 μM for benzyl ester ()-16a). For anionically functionalizedreceptors 12, 13, and 17 the ChT inhibition is basedessentially on electrostatic interaction, and the bound enzymecan be released from the resorcarene surface by increasing the ionic strength, with its activity almost completely restored. Forreceptors with terminal benzyl ester groups (10 and 16) a hydrophobic network can be suggested.
Description: 
19589
URI: http://hdl.handle.net/20.500.12779/5998
ISSN: 0022-3263
DOI: 10.1021/jo102592f
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