Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5979
Title: Structure of the extended-spectrum beta-lactamase TEM-72 inhibited by citrate
Authors: Docquier, JEAN DENIS
Benvenuti, Manuela 
V., Calderone
Rossolini, GIAN MARIA
Mangani, Stefano 
Keywords: x-ray; crystal structure; beta-lactamase; tem-72
Issue Date: 2011
Project: None 
Journal: ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
Abstract: 
TEM-72, a class A beta-lactamase identified in isolates of Enterobacteriaceae, is a quadruple mutant of TEM-1 (Q39K, M182T, G238S and E240K) and shows extended-spectrum beta-lactamase (ESBL) properties arising from the G238S and E240K substitutions. Although many structures of TEM variants have been published, they do not include an enzyme with the simultaneous presence of both of the ESBL-conferring G238S and E240K substitutions. Furthermore, the structure shows the presence of a citrate anion bound to the TEM-72 active site, where it interacts with all of the conserved residues of class A beta-lactamases. The present structure supports the use of polycarboxylates as a scaffold for the design of broad-spectrum inhibitors of serine beta-lactamases.
Description: 
47916
URI: http://hdl.handle.net/20.500.12779/5979
ISSN: 1744-3091
DOI: 10.1107/S1744309110054680
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