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Title: Structural features of apramycin bound at the bacterial ribosome a site as detected by NMR and CD spectroscopy
Authors: Balenci, D
D'Amelio, N
Gaggelli, Elena 
Gaggelli, Nicola 
Cellai, L
Molteni, E
Valensin, Gianni 
Issue Date: 2010
Project: None 
The interaction of apramycin with an RNA fragment mimicking the bacterial ribosomal A site was investigated by NMR spectroscopy, mainly by using the transferred NOE technique. The fact that only the RNA-bound antibiotic shows nonzero NOE effects, allowed us to gain structural details of bound apramycin despite the fast exchange conditions between the free and RNA-bound forms. The dissociation constant of the apramycin–RNA complex was evaluated by CD spectroscopy.
ISSN: 1439-4227
DOI: 10.1002/cbic.200900629
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