Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5772
DC FieldValueLanguage
dc.contributor.authorBraconi, Danielaen_us
dc.contributor.authorLaschi, Marcellaen_us
dc.contributor.authorTaylor, Aen_us
dc.contributor.authorBernardini, Giuliaen_us
dc.contributor.authorSpreafico, Adrianoen_us
dc.contributor.authorTinti, Len_us
dc.contributor.authorGallagher, Jen_us
dc.contributor.authorSantucci, Annalisaen_us
dc.date.accessioned2021-03-30T15:55:01Z-
dc.date.available2021-03-30T15:55:01Z-
dc.date.issued2010-
dc.identifier.issn0730-2312en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12779/5772-
dc.description24880en_US
dc.description.abstractAlkaptonuria (AKU) is a rare genetic disease associated with the accumulation of homogentisic acid (HGA) and its oxidized/polymerized products in connective tissues up to the deposition of melanin-like pigments (ochronosis). Since little is known on the effects of HGA and its metabolites on articular cells, we carried out a proteomic and redox-proteomic analysis to investigate how HGA and ascorbic acid (ASC) affect the human chondrocytic protein repertoire. We settled up an in vitro model using a human chondrocytic cell line to evaluate the effects of 0.33 mM HGA, alone or combined with ASC. We found that HGA and ASC significantly affect the levels of proteins with specific functions in protein folding, cell organization and, notably, stress response and cell defense. Increased protein carbonyls levels were found either in HGA or ASC treated cells, and evidences produced in this paper support the hypothesis that HGA-induced stress might be mediated by protein oxidation. Our finding can lay the basis towards the settling up of more sophisticated models to study AKU and ochronosis.en_US
dc.language.isoenen_US
dc.relationNoneen_US
dc.relation.ispartofJOURNAL OF CELLULAR BIOCHEMISTRYen_US
dc.titleProteomic and redox-proteomic evaluation of homogentisic acid and ascorbic acid effects on human articular chondrocytesen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/jcb.22780en_US
dc.identifier.pmid20665660en_US
dc.identifier.scopus2-s2.0-78049247544en_US
dc.identifier.isiWOS:000283960100018en_US
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jcb.22780/abstracten_US
dc.relation.volume111(4)en_US
dc.description.firstpage922en_US
dc.description.lastpage932en_US
dc.description.thirdmissionNot applicableen_US
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.orcid0000-0002-9657-4169-
crisitem.author.orcid0000-0003-0016-0728-
crisitem.author.orcid0000-0001-6976-9086-
Appears in Collections:Publications
Show simple item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.