Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5660
DC FieldValueLanguage
dc.contributor.authorD., Balencien_us
dc.contributor.authorG., Bonechien_us
dc.contributor.authorN., D'Amelioen_us
dc.contributor.authorGaggelli, Elenaen_us
dc.contributor.authorGaggelli, Nicolaen_us
dc.contributor.authorE., Moltenien_us
dc.contributor.authorValensin, Giannien_us
dc.contributor.authorW., Szczepaniken_us
dc.contributor.authorM., Dziubaen_us
dc.contributor.authorG., Swiecickien_us
dc.contributor.authorM., JEZOWSKA BOJCZUKen_us
dc.date.accessioned2021-03-30T15:54:12Z-
dc.date.available2021-03-30T15:54:12Z-
dc.date.issued2009-
dc.identifier.issn1477-9226en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12779/5660-
dc.description35601en_US
dc.description.abstractThe interaction of apramycin with copper at different pH values was investigated by potentiometric titrations and EPR, UV-vis and CD spectroscopic techniques. The Cu(II)-apramycin complex prevailing at pH 6.5 was further characterized by NMR spectroscopy. Metal-proton distances derived from paramagnetic relaxation enhancements were used as restraints in a conformational search procedure in order to define the structure of the complex. Longitudinal relaxation rates were measured with the IR-COSY pulse sequence, thus solving the problems due to signal overlap. At pH 6.5 apramycin binds copper(II) with a 2 : 1 stoichiometry, through the vicinal hydroxyl and deprotonated amino groups of ring III. Plasmid DNA electrophoresis showed that the Cu(II)-apramycin complex is more active than free Cu(II) in generating strand breakages. Interestingly, this complex in the presence of ascorbic acid damages DNA with a higher yield than in the presence of H2O2.en_US
dc.language.isoenen_US
dc.relationNoneen_US
dc.relation.ispartofDALTON TRANSACTIONSen_US
dc.titleStructural features and oxydative stress towards plasmid DNA of apramycin copper complexen_US
dc.typeArticleen_US
dc.identifier.doi10.1039/b815046jen_US
dc.identifier.scopus2-s2.0-59349087351en_US
dc.identifier.isiWOS:000263112900008en_US
dc.relation.volume2009en_US
dc.description.firstpage1123en_US
dc.description.lastpage1130en_US
dc.description.thirdmissionNot applicableen_US
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.orcid9810-
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