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|Title:||Interaction of Quercetin and its conjugate Quercetin 3-b-D-glucoside with Albumin Determined by NMR Relaxation Data||Authors:||Martini, S
|Keywords:||NMR Relaxation Data; Quercetin||Issue Date:||2008||Project:||None||Journal:||JOURNAL OF NATURAL PRODUCTS||Abstract:||
NMR methodology has been developed in order to study phytochemical-macromolecular receptor interactions. This approach is based on the analysis of proton selective spin–lattice relaxation rate enhancements of the ligand in the presence of the macromolecule, to calculate an afﬁnity index, [A] , related to the strength of the interaction process. This index has been modiﬁed by normalization to the relaxation rate of the free ligand, in order to take into account the effects of motional anisotropies and different proton densities. The normalized afﬁnity index, [A L T N ] , isolates the contribution due to a decrease in the ligand dynamics caused by the binding with the protein. This methodology has been applied to the interaction between two ﬂavonoids (quercetin, 1, and quercetin 3-O-D-glucopyranoside, 2) and bovine serum albumin (BSA). The calculated values of the afﬁnity indexes and thermodynamic equilibrium constants suggested a much stronger capacity of 1 to interact with BSA when compared with its glucosylated derivative, 2.
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