Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5461
Title: Morphogenesis of human endothelial cells is inhibited by DAB2 via Src.
Authors: Orlandini, Maurizio 
Nucciotti, S
Galvagni, Federico 
Bardelli, M
Rocchigiani, Marina
Petraglia, Felice
Oliviero, Salvatore 
Issue Date: 2008
Project: None 
Journal: FEBS LETTERS
Abstract: 
Disabled-2 (DAB2) is an adaptor protein implicated in signal transduction pathways and in protein traffic regulation. Here, we show that DAB2 is highly expressed in human endothelial cells. DAB2 silencing in endothelial cells by lentiviral-mediated small hairpin RNA expression affects cell migration and differentiation into capillary-like structures while increasing cell proliferation and viability. DAB2 knockdown causes activation of the Src-FAK signal pathway, extracellular-signal regulated kinase and c-Jun NH2-terminal kinase activation, and inhibition of p38 phosphorylation. In DAB2 silenced endothelial cells, pharmacological inhibition of Src with its specific inhibitor PP2 abolishes focal adhesion kinase activation and restores differentiation of endothelial cells. These results suggest that DAB2, via Src and focal adhesion signaling, plays a role in human endothelial cell function.
Description: 
35708
URI: http://hdl.handle.net/20.500.12779/5461
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2008.06.025
Appears in Collections:Publications

Show full item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.