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|Title:||Identification and structural characterization of a transient Radical Species in the Uricase Reaction Mechanism||Authors:||Busi, Elena
|Issue Date:||2007||Project:||None||Journal:||APPLIED MAGNETIC RESONANCE||Abstract:||
Uricase catalyzes the oxidation of urate to form allantoin, carbon dioxide and hydrogen peroxide. In this article, we demonstrate for the first time the presence of a radical intermediate involved in the reaction mechanism. Such radical species was entrapped using 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide as spin trap and the relative adduct was detected by electron paramagnetic resonance (EPR) technique. A structure of such radical (5-hydroperoxy isourate) is proposed, through chemical results and density functional theory calculations of the EPR coupling constants.
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