Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5362
Title: Identification and structural characterization of a transient Radical Species in the Uricase Reaction Mechanism
Authors: Busi, Elena 
Sinicropi, Adalgisa 
Terzuoli, L.
Marinello, E.
Basosi, Riccardo 
Issue Date: 2007
Project: None 
Journal: APPLIED MAGNETIC RESONANCE
Abstract: 
Uricase catalyzes the oxidation of urate to form allantoin, carbon dioxide and hydrogen peroxide. In this article, we demonstrate for the first time the presence of a radical intermediate involved in the reaction mechanism. Such radical species was entrapped using 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide as spin trap and the relative adduct was detected by electron paramagnetic resonance (EPR) technique. A structure of such radical (5-hydroperoxy isourate) is proposed, through chemical results and density functional theory calculations of the EPR coupling constants.
Description: 
36282
URI: http://hdl.handle.net/20.500.12779/5362
ISSN: 0937-9347
DOI: 10.1007/BF03166597
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