Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5350
Title: Crystallization of soluble proteins in vapor diffusion for x-ray crystallography
Authors: Benvenuti, Manuela 
Mangani, Stefano 
Keywords: crystallization; protein crystallography
Issue Date: 2007
Project: None 
Journal: NATURE PROTOCOLS
Abstract: 
The preparation of protein single crystals represents one of the major obstacles in obtaining the detailed 3D structure of a biological macromolecule. The complete automation of the crystallization procedures requires large investments in terms of money and labor, which are available only to large dedicated infrastructures and is mostly suited for genomic-scale projects. On the other hand, many research projects from departmental laboratories are devoted to the study of few specific proteins. Here, we try to provide a series of protocols for the crystallization of soluble proteins, especially the difficult ones, tailored for small-scale research groups. An estimate of the time needed to complete each of the steps described can be found at the end of each section.
Description: 
47923
URI: http://hdl.handle.net/20.500.12779/5350
ISSN: 1754-2189
DOI: 10.1038/nprot.2007.198
Appears in Collections:Publications

Show full item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.