Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5319
Title: Analysis of guazatine mixture by Lc and LC-MS and antimycotic activity determination of principal components
Authors: Dreassi, Elena 
Zizzari, ALESSANDRA TANIA
D'Arezzo, S
Visca, P
Botta, Maurizio 
Keywords: HPLC/UV/MS, Guazatine, Candida, Antimycotic activity, MICs
Issue Date: 2007
Project: None 
Journal: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Abstract: 
iminodi(octamethylene)diamine, octamethylenebis(imino-octamethylene) diamine and carbamonitrile. In this work, the analysis of guazatine mixture by LC and LC–MS has been treated for the first time. In the guazatine mixture diamine derivatives account for 40% of the constituents of guazatine, triamines for 46%, tetramines for 11% and other amine derivatives for 3%. The most abundant individual components are the fully guanidated triamine (GGG, 30.6%) and the fully guanidated diamine (GG, 29.5%) followed by the monoguanidated diamine (GN, 9.8%) and a diguanidated triamine (GGN, 8.1%). The identification and separation of main components of commercial guazatine was performed through a new LC–MS method. The separation was performed on an Alltima C18 column using linear gradient elution (formic acid in water and acetonitrile) with UV-detection at 200 nm and the identification was performed by ESI+-mass spectrometry analysis. The main components (GN, GG, GNG, GGN, GGG and GGGG) were then purified and separated from the mixture. Antimycotic activity of guazatine derivatives was determined on different species and strains belonging to genus Candida. The results obtained suggest that GNG and GGGG components can further be developed in new antifungal compounds with high potential for the treatment of Candida infections.
Description: 
36809
URI: http://hdl.handle.net/20.500.12779/5319
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2006.10.029
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