Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5157
DC FieldValueLanguage
dc.contributor.authorMini, Ren_us
dc.contributor.authorAnnibale, Ben_us
dc.contributor.authorLahner, Een_us
dc.contributor.authorBernardini, Giuliaen_us
dc.contributor.authorFigura, Nataleen_us
dc.contributor.authorSantucci, Annalisaen_us
dc.date.accessioned2021-03-30T15:49:29Z-
dc.date.available2021-03-30T15:49:29Z-
dc.date.issued2006-
dc.identifier.issn0009-9147en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12779/5157-
dc.description24882en_US
dc.description.abstractBackground: Atrophic body gastritis is considered the first important step in the histogenesis of gastric carcinoma, a multistep process starting from chronic gastritis and progressing through chronic atrophic gastritis, intestinal metaplasia, and dysplasia. Helicobacter pylori is involved in the induction of atrophic body gastritis, but documentation of H. pylori infection is difficult because of the progressive disappearance of the bacterium. Our study aimed to detect past H. pylori infection in patients with atrophic body gastritis. Methods: We used Western blot analyses of whole bacterial protein lysate of 2 different strains to probe sera from 143 patients. All sera were analyzed by ELISA (Bio-Rad), and results of gastric histology were available for all patients. Results: Among 111 patient sera previously classified as negative for H. pylori infection by ELISA, 106 (95.5%) were positive when assayed by immunoblotting. Conclusions: Commercial diagnostic reagent sets may fail to detect H. pylori infection. Western blotting of whole bacterial protein extracts could provide the basis of a noninvasive serology tool able to assess previous infection with H. pylori in patients with atrophic body gastritis.en_US
dc.language.isoenen_US
dc.relationNoneen_US
dc.relation.ispartofCLINICAL CHEMISTRYen_US
dc.titleWestern blotting of total lysate of Helicobacter pylori in cases of atrophic body gastritis.en_US
dc.typeArticleen_US
dc.identifier.doi10.1373/clinchem.2005.054627en_US
dc.identifier.pmid16306089en_US
dc.identifier.scopus2-s2.0-31844442368en_US
dc.identifier.isiWOS:000235069600007en_US
dc.identifier.urlhttp://www.clinchem.org/content/52/2/220.longen_US
dc.relation.volume52en_US
dc.relation.issue2en_US
dc.description.firstpage220en_US
dc.description.lastpage226en_US
dc.description.thirdmissionNot applicableen_US
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.orcid0000-0003-0016-0728-
crisitem.author.orcid0000-0001-6976-9086-
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