Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5152
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dc.contributor.authorSpiga, Ottaviaen_us
dc.contributor.authorPadula, Mgen_us
dc.contributor.authorScarselli, Men_us
dc.contributor.authorCiutti, Aen_us
dc.contributor.authorBernini, Andreaen_us
dc.contributor.authorVenditti, Ven_us
dc.contributor.authorPrischi, Fen_us
dc.contributor.authorFalciani, Chiaraen_us
dc.contributor.authorLozzi, Luisaen_us
dc.contributor.authorBracci, Luisaen_us
dc.contributor.authorValensin, Peen_us
dc.contributor.authorCaudai, Cen_us
dc.contributor.authorNiccolai, Nerien_us
dc.date.accessioned2021-03-30T15:49:27Z-
dc.date.available2021-03-30T15:49:27Z-
dc.date.issued2006-
dc.identifier.issn1359-6535en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12779/5152-
dc.description37150en_US
dc.description.abstractA structural genomics approach is proposed for the development of new diagnostic kits. It combines molecular modelling, peptide synthesis and immunological tests. The preliminary step is the development of a reliable three-dimensional structure of an immunodominant protein of the target pathogenic organism using the various bioinformatic strategies that are now available to structural biologists. Once the protein structure is obtained, the most surface-exposed fragments with minimal sequence variability among the different strains reported in the genomic data bank are reproduced synthetically as linear peptides. These peptides are then tested for immunoreactivity with the plasma of infected patients to determine whether the synthetic molecules have antigenic activity and can therefore be used to detect infecting agents. This structurally driven selection of mimotopes was successfully performed for the human hepatitis C virus, as five peptides that specifically interact with the plasma of HCV-infected patients were identified solely on the basis of the three-dimensional structure predicted for the E2 homodimer of the la viral subtype. A similar approach could easily be extended to a large variety of immunogenic proteins from other pathogenic organisms.en_US
dc.language.isoenen_US
dc.relationNoneen_US
dc.relation.ispartofANTIVIRAL THERAPYen_US
dc.titleStructurally driven selection of human hepatitis C virus mimotopes.en_US
dc.typeArticleen_US
dc.identifier.pmid17302254en_US
dc.identifier.scopus2-s2.0-33750940548en_US
dc.identifier.isiWOS:000241975800010en_US
dc.relation.volume11en_US
dc.relation.issue7en_US
dc.description.firstpage917en_US
dc.description.lastpage922en_US
dc.description.thirdmissionNot applicableen_US
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.orcid0000-0002-0263-7107-
crisitem.author.orcid0000-0002-7528-2749-
crisitem.author.orcid0000-0002-6919-6908-
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