Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5152
Title: Structurally driven selection of human hepatitis C virus mimotopes.
Authors: Spiga, Ottavia 
Padula, Mg
Scarselli, M
Ciutti, A
Bernini, Andrea 
Venditti, V
Prischi, F
Falciani, Chiara
Lozzi, Luisa
Bracci, Luisa
Valensin, Pe
Caudai, C
Niccolai, Neri 
Issue Date: 2006
Project: None 
Journal: ANTIVIRAL THERAPY
Abstract: 
A structural genomics approach is proposed for the development of new diagnostic kits. It combines molecular modelling, peptide synthesis and immunological tests. The preliminary step is the development of a reliable three-dimensional structure of an immunodominant protein of the target pathogenic organism using the various bioinformatic strategies that are now available to structural biologists. Once the protein structure is obtained, the most surface-exposed fragments with minimal sequence variability among the different strains reported in the genomic data bank are reproduced synthetically as linear peptides. These peptides are then tested for immunoreactivity with the plasma of infected patients to determine whether the synthetic molecules have antigenic activity and can therefore be used to detect infecting agents. This structurally driven selection of mimotopes was successfully performed for the human hepatitis C virus, as five peptides that specifically interact with the plasma of HCV-infected patients were identified solely on the basis of the three-dimensional structure predicted for the E2 homodimer of the la viral subtype. A similar approach could easily be extended to a large variety of immunogenic proteins from other pathogenic organisms.
Description: 
37150
URI: http://hdl.handle.net/20.500.12779/5152
ISSN: 1359-6535
Appears in Collections:Publications

Show full item record

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.