Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5094
Title: The crystal structure of yeast copper thionein: the solution of a long-lasting enigma
Authors: Calderone, Vito
Dolderer, B.
Hartmann, H. J.
Echner, H.
Luchinat, C.
DEL BIANCO, C.
Mangani, Stefano 
Weser, U.
Keywords: copper metabolism; metallothionein; Saccharomyces cerevisiae; x-ray structure
Issue Date: 2005
Project: None 
Journal: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Abstract: 
We report here the crystal structure of yeast copper thionein (Cu-MT), determined at 1.44-A resolution. The Cu-MT structure shows the largest known oligonuclear Cu(I) thiolate cluster in biology, consisting of six trigonally and two digonally coordinated Cu(I) ions. This is at variance with the results from previous spectroscopic determinations, which were performed on MT samples containing seven rather than eight metal ions. The protein backbone has a random coil structure with the loops enfolding the copper cluster, which is located in a cleft where it is bound to 10 cysteine residues. The protein structure is somewhat different from that of Ag(7)-MT and similar, but not identical, to that of Cu(7)-MT. Besides the different structure of the metal cluster, the main differences lie in the cysteine topology and in the conformation of some portions of the backbone. The present structure suggests that Cu-MT, in addition to its role as a safe depository for copper ions in the cell, may play an active role in the delivery of copper to metal-free chaperones.
Description: 
21826
URI: http://hdl.handle.net/20.500.12779/5094
ISSN: 0027-8424
DOI: 10.1073/pnas.0408254101
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