Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/5076
Title: NMR Studies on Cu(II)-Peptide Complexes: Exchange Kinetics and Determination of Structures in Solution
Authors: Gaggelli, Elena 
H., Kozlowski
Valensin, Daniela 
Valensin, Gianni 
Issue Date: 2005
Project: None 
Journal: MOLECULAR BIOSYSTEMS
Abstract: 
The interaction of copper(II) with histidine containing peptides has recently acquired renewed interest following the established link between abnormal protein behaviour in neurodegenerative processes and unpaired copper homeostasis. Five peptide sequences taken from the amyloid precursor protein and the prion protein were considered. Addition of paramagnetic Cu(II) ions to solutions of such peptides was not found to severely affect the appearance of NMR spectra, thus limiting the usual approach for structural determination. Exchange kinetics was shown to play a major role in determining the observed paramagnetic spin-lattice relaxation rates. Two independent methods were suggested for evaluating the exchange rates of His-containing peptides from the copper-coordination sphere and to calculate copper–proton distances. In such a way NMR was demonstrated to have the potential of providing detailed structures of the Cu(II)–peptide complexes in solution.
Description: 
36345
URI: http://hdl.handle.net/20.500.12779/5076
ISSN: 1742-206X
DOI: 10.1039/b419413f
Appears in Collections:Publications

Show full item record

Page view(s)

338
Last Week
78
Last month
69
checked on May 11, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.