Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4956
Title: EPR Spin Trapping of a radical intermediate in the urate oxidase reaction
Authors: Busi, Elena 
Terzuoli, Lucia
Basosi, Riccardo 
Porcelli, Brunetta
Marinello, Enrico
Keywords: uric acid; oxidation; Uricase; spin-trap; radical intermediate
Issue Date: 2004
Project: None 
Journal: NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS
Abstract: 
Urate oxidase, or uricase (EC 1.7.3.3), is a peroxisomal enzyme that catalyses the oxidation of uric acid to allantoin. The chemical mechanism of the urate oxidase reaction has not been clearly established, but the involvement of radical intermediates was hypothesised. In this study EPR spectroscopy by spin trapping of radical intermediates has been used in order to demonstrate the eventual presence of radical transient urate species. The oxidation reaction of uric acid by several uricases (Porcine Liver, Bacillus Fastidiosus, Candida Utilitis) was performed in the presence of 5‐diethoxyphosphoryl‐5‐methyl‐pyrroline‐N‐oxide (DEPMPO) as spin trap. DEPMPO was added to reaction mixture and a radical adduct was observed in all cases. Therefore, for the first time, the presence of a radical intermediate in the uricase reaction was experimentally proved.
Description: 
35564
URI: http://hdl.handle.net/20.500.12779/4956
ISSN: 1525-7770
DOI: 10.1081/NCN-200027390
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