Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4951
DC FieldValueLanguage
dc.contributor.authorA., Zippoen_us
dc.contributor.authorA., DE ROBERTISen_us
dc.contributor.authorM., Bardellien_us
dc.contributor.authorGalvagni, Federicoen_us
dc.contributor.authorS., Olivieroen_us
dc.date.accessioned2021-03-30T15:45:49Z-
dc.date.available2021-03-30T15:45:49Z-
dc.date.issued2004-
dc.identifier.issn0006-4971en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12779/4951-
dc.description37972en_US
dc.description.abstractThe tyrosine kinase receptor fetal liver kinase 1 (Flk-1) plays a crucial role in vasculogenesis and angiogenesis, but its target genes remain elusive. Comparing Flk-1(+/+) with Flk-1(-/-) embryonic stem (ES) cells, we identified transcripts regulated by the vascular endothelial growth factor A (VEGF-A)/Flk-1 pathway at an early stage of their differentiation to endothelial and mural precursors. Further analysis of a number of these genes (Nm23-M1, Nm23-M2, Slug, Set, pp32, Cbp, Ship-1, Btk, and Pim-1) showed that their products were transiently up-regulated in vivo in endothelial cells (ECs) during angiogenesis of the ovary, and their mRNA was rapidly induced in vitro by VEGF-A in human umbilical cord vein endothelial cells (HUVECs). Functional analysis by RNA interference (RNAi) in ES cells induced to differentiate demonstrated that Pim-1 is required for their differentiation into ECs and smooth muscle cells (SMCs). In HUVECs, RNAi showed that Pim-1 is required in ECs for VEGF-A-dependent proliferation and migration. The identification of Flk-1 target genes should help in elucidating the molecular pathways that govern the vasculogenesis and angiogenesis processes.en_US
dc.language.isoenen_US
dc.relationNoneen_US
dc.relation.ispartofBLOODen_US
dc.titleIdentification of Flk-1-target genes in vasculogenesis: Pim-1 is required for endothelial and mural cell differentiation in vitro.en_US
dc.typeArticleen_US
dc.identifier.doi10.1182/blood-2003-11-3827en_US
dc.identifier.pmid14982870en_US
dc.identifier.scopus2-s2.0-2942525073en_US
dc.identifier.isiWOS:000222001700033en_US
dc.identifier.urlhttp://bloodjournal.hematologylibrary.org/content/103/12/4536.longen_US
dc.relation.volume103en_US
dc.description.firstpage4536en_US
dc.description.lastpage4544en_US
dc.description.thirdmissionNot applicableen_US
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.orcid0000-0003-1967-9554-
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