Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4947
Title: Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism
Authors: S., Benini
W. R., Rypniewski
K. S., Wilson
Mangani, Stefano 
S., Ciurli
Keywords: urease; x-ray structure; catalytic mechanism; inhibitor
Issue Date: 2004
Project: None 
Journal: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Abstract: 
The structure of the complex of urease, a Ni-containing metalloenzyme, with boric acid was determined at 2.10 A resolution. The complex shows the unprecedented binding mode of the competitive inhibitor to the dinuclear metal center, with the B(OH)3 molecule bridging the Ni ions and leaving in place the bridging hydroxide. Boric acid can be considered a substrate analogue of urea, and the structure supports the proposal that the Ni-bridging hydroxide acts as the nucleophile in the enzymatic process of urea hydrolysis.
Description: 
21807
URI: http://hdl.handle.net/20.500.12779/4947
ISSN: 0002-7863
DOI: 10.1021/ja049618p
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