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Title: Physiological T cell activation starts and propagates in lipid rafts.
Authors: Pizzo, P.
Giurisato, Emanuele 
Bigsten, A.
Tassi, M.
Tavano, R.
Shaw, A. S.
Viola, A.
Issue Date: 2004
Project: None 
Lipid rafts are plasma membrane compartments enriched in key signaling molecules. We have previously shown that in T lymphocytes anti-CD3 stimulation is insensitive to cholesterol extraction by methyl-beta-cyclodextrin (MbetaCD), suggesting that anti-CD3 induced signal transduction is independent of raft integrity. Here we show that, in contrast to T cell stimulation by anti-CD3 antibodies, T cell activation by a physiological ligand is mediated by signaling events taking place in lipid raft. Indeed, cholesterol depletion by MbetaCD resulted in reduced T cell activation in response to Epstein Barr Virus (EBV)-transformed B cells pulsed with a bacterial superantigen. Moreover, T cell stimulation by pulsed EBV-B cells, but not by anti-CD3 antibodies, induced recruitment of active Lck in detergent-resistant membranes, where the signal transduction is organized and amplified.
ISSN: 0165-2478
DOI: 10.1016/j.imlet.2003.09.008
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