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Title: In vivo C-13-NMR and modelling study of metabolic yield response to ethanol stress in a wild-type strain of Saccharomyces cerevisiae
Authors: Martini, Silvia
Ricci, Maso
Bonechi, Claudia 
Trabalzini, Lorenza 
Santucci, Annalisa 
Rossi, Claudio 
Keywords: metabolism; modelling; inhibition; in vivo nuclear magnetic resonance; Saccharomyces cerevisiae
Issue Date: 2004
Project: None 
In this paper the combined use of in vivo (13)C-nuclear magnetic resonance spectroscopy and mathematical modelling allowed the analysis of the response to ethanol stress in a wild-type strain of Saccharomyces cerevisiae, in terms of a reduced metabolic activity. The model developed succeeded in describing and interpreting the effects of increasing concentrations of exogenous ethanol. In particular, the ratio between the kinetic constants associated with ethanol production and glucose consumption gave the estimation of the metabolic yield of the processes in perfect agreement with experimental results.
ISSN: 0014-5793
DOI: 10.1016/S0014-5793(04)00316-3
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