Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4902
Title: Solution structure of Cox11, a novel type of beta-immunoglobulin-like fold involved in CuB site formation of cytochrome c oxidase
Authors: Banci, L.
Bertini, I.
Cantini, F.
CIOFI BAFFONI, S.
Gonnelli, L.
Mangani, Stefano 
Keywords: copper transport; cytochrome oxidase; NMR; X-ray absorption
Issue Date: 2004
Project: None 
Journal: THE JOURNAL OF BIOLOGICAL CHEMISTRY
Abstract: 
Cytochrome c oxidase assembly process involves many accessory proteins including Cox11, which is a copper-binding protein required for Cu incorporation into the Cu(B) site of cytochrome c oxidase. In a genome wide search, a number of Cox11 homologs are found in all of the eukaryotes with complete genomes and in several Gram-negative bacteria. All of them possess a highly homologous soluble domain and contain an N-terminal fragment that anchors the protein to the membrane. An anchor-free construct of 164 amino acids was obtained from Sinorhizobium meliloti, and the first structure of this class of proteins is reported here. The apoform has an immunoglobulin-like fold with a novel type of beta-strand organization. The copper binding motif composed of two highly conserved cysteines is located on one side of the beta-barrel structure. The apoprotein is monomeric in the presence of dithiothreitol, whereas it dimerizes in the absence of the reductant. When copper(I) binds, NMR and extended x-ray absorption fine structure (EXAFS) data indicate a dimeric protein state with two thiolates bridging two copper(I) ions. The present results advance the knowledge on the poorly understood molecular aspects of cytochrome c oxidase assembly.
Description: 
21808
URI: http://hdl.handle.net/20.500.12779/4902
ISSN: 0021-9258
DOI: 10.1074/jbc.M403655200
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