Please use this identifier to cite or link to this item:
|Title:||Chalcone Prenyltransferase from Morus nigra cell cultures. Substrate specificity studies||Authors:||Vitali, A.
DELLE MONACHE, G.
|Issue Date:||2004||Project:||None||Journal:||FEBS LETTERS||Abstract:||
A new prenyltransferase (PT) enzyme derived from the microsomal fractions of cell cultures of Morus nigra was shown to be able to prenylate exclusively chalcones with a 2',4'-dihydroxy substitution and the isoflavone genistein. Computational studies were performed to shed some light on the relationship between the structure of the substrate and the enzymatic activity. PT requires divalent cations, particularly Mg2+, to be effective. The apparent K-m values for gamma,gamma-dimethylallyldiphosphate and 2',4'-dihydroxychalcone were 63 and 142 muM, respectively. The maximum activity of the enzyme was expressed during the first 10 days of cell growth.
|Appears in Collections:||Publications|
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.