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|Title:||The effect of exposure to high flux density static and pulsed magnetic fields on lymphocyte function||Authors:||Aldinucci, Carlo
BLANCO GARCIA, J.
Sgaragli, GIAN PIETRO
Pessina, GIAN PAOLO
|Keywords:||NMR fields; magnetic resonance; calcium; proliferation; cytokines||Issue Date:||2003||Project:||None||Journal:||BIOELECTROMAGNETICS||Abstract:||
We investigated whether a combination of static electromagnetic field (EMF) at a flux density of 4.75Ttogether with pulsed EMF at a flux density of 0.7 mT generated by an NMR apparatus (NMRF), couldpromote movements of Ca2þ, cell proliferation, and the eventual production of proinflammatorycytokines in human lymphocytes as well as in Jurkat cells, after exposure to the field for 1 h. The samestudy was also performed after activation of cells with 5 mg/ml phytohaemagglutinin (PHA)immediately before the exposure period. Our results clearly demonstrate that NMRF exposure increasesthe [Ca2þ]i, without any proliferative, or activating, or proinflammatory effect on both normaland PHA stimulated lymphocytes. Accordingly, the levels of interferon g, tumor necrosis factor a,interleukin-1b, interleukin-2, and interleukin-6 remained unvaried after exposure. Exposure of Jurkatcells statistically decreased the [Ca2þ]i and the proliferation. This is consistent with the low levels ofIL-2 measured in supernatants of these cells after exposure. On the whole our data suggest that staticand pulsed NMRF exposure contribute synergistically in the increase of the [Ca2þ]i without anyactivating or proinflammatory effect either in normal or in PHA challenged lymphocytes. In Jurkatcells, by changing the properties of cell membranes, NMRF exposure can influence Ca2þ transportprocesses and hence Ca2þ homeostasis, causing a marked decrease of proliferation.
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