Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4815
Title: XAS of dilute biological samples
Authors: M., Ranieri Raggi
A., Raggi
D., Martini
M., Benvenuti
Mangani, Stefano 
Keywords: XAS; adenosine monophosphate deaminase; dilution
Issue Date: 2003
Project: None 
Journal: JOURNAL OF SYNCHROTRON RADIATION
Abstract: 
The experimental setup of beamline ID26 at ESRF (Grenoble) has been successfully exploited to obtain high-quality XAS (X-ray absorption spectroscopy) data from a biological sample where the metal concentration is about 100 muM. The sample consists of the adenosine monophosphate deaminase (AMPD) histidine proline rich glycoprotein (HPRG) complex that contains 3-4 Zn(II) ions per dimer of approximately 320 kDa molecular weight. The experiment shows that third-generation X-ray sources equipped with insertion devices and appropriate optics and detectors allow the investigation of complex biological systems where the metal concentration is intrinsically low. The availability of such experimental setups makes possible a completely new set of experiments in biological XAS.
Description: 
47951
URI: http://hdl.handle.net/20.500.12779/4815
ISSN: 0909-0495
DOI: 10.1107/S0909049502017260
Appears in Collections:Publications

Show full item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.