Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4812
Title: Cu(II) ion coordination to unprotected pentadecapeptide contaning two His residues: competition between the terminal amino and the side-chain imidazole nitrogen donors
Authors: Conato, C
Kamysz, W
Kozlowski, H
Luczkowski, M
Mackiewicz, Z
Mancini, F
Mlynarz, P
Remelli, M
Valensin, Daniela 
Valensin, Gianni 
Keywords: MATRICELLULAR PROTEIN; COPPER-COMPLEXES; CELL-MATRIX; SPARC; PEPTIDES; EXCITATION; STABILITY; LIGANDS
Issue Date: 2003
Project: None 
Journal: EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
Abstract: 
The complex-formation equilibria of the pentadecapeptide TLEGTKKGHKLHLDY, the 114-128 protein fragment of SPARC, with the Cull ion have been investigated, at I = 0.1 mol.dm(-3) (KNO3) and T = 298.2 K. Protonation and complex-formation constants have been determined potentiometrically, and formation enthalpies measured by direct solution calorimetry; the complex-formation model and species stoichiometry have been carefully checked by means of UV/Vis absorption, CD and EPR spectroscopy. The structure hypo-theses of the complex species are also based on detailed study of the H-1 and C-13 NMR spectra of the ligand in both the absence and presence of copper ions. The involvement in complex-formation of both the terminal amino and imidazole groups has been suggested and their specific behaviour at different pH values elucidated.
Description: 
27139
URI: http://hdl.handle.net/20.500.12779/4812
ISSN: 1434-1948
DOI: 10.1002/ejic.200200551
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