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|Title:||Micropatterned polysaccharide surfaces via laser ablation for cell guidance.||Authors:||Barbucci, Rolando
|Keywords:||hyaluronic acid; photoimmobilization; laser ablation; cell behaviour; fibrobblasts; endothelial cells||Issue Date:||2003||Project:||None||Journal:||MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS||Abstract:||
Micropatterned materials were obtained by a controlled laser ablation of a photoimmobilised homogeneous layer of hyaluronic acid(Hyal) and its sulphated derivative (HyalS). The photoimmobilisation was performed by coating the polysaccharide, adequatelyfunctionalised with a photoreactive group, on aminosilanised glass substrate and immobilising it on the surface under UV light. Hyal orHyalS photoimmobilised samples were then subjected to laser ablation with wavelengths in the UV regions in order to drill the pattern. Fourdifferent patterns with stripes of 100, 50, 25 and 10 Am were generated. A chemical characterisation by attenuated total reflection/Fouriertransform infrared (ATR/FT-IR) and time of flight-secondary ions mass spectrometry (TOF-SIMS) confirmed the success of the laser ablationprocedure and the presence of alternating stripes of polysaccharide and native glass. The exact dimensions of the stripes were determined byatomic force microscopy. The analysis of cell behaviour in terms of adhesion, proliferation and movement using mouse fibroblasts (3T3 line)and bovine aortic endothelial cells (BAEC) was also performed.
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