Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4687
DC FieldValueLanguage
dc.contributor.authorPizzo, P.en_us
dc.contributor.authorGiurisato, Emanueleen_us
dc.contributor.authorTassi, M.en_us
dc.contributor.authorBenedetti, A.en_us
dc.contributor.authorPozzan, T.en_us
dc.contributor.authorViola, A.en_us
dc.date.accessioned2021-03-30T15:43:57Z-
dc.date.available2021-03-30T15:43:57Z-
dc.date.issued2002-
dc.identifier.issn0014-2980en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12779/4687-
dc.description37616en_US
dc.description.abstractThe current model suggesting that raft integrity is required for T cell activation is mostly (but not exclusively) based on the use of drugs, such as methyl-beta-cyclodextrin (M beta CD), that disorganize rafts and inhibit T cell receptor (TCR)-induced Ca2+ influx. Here we show that conditions that disrupt lipid raft integrity do not inhibit TCR triggering in Jurkat cells and normal T lymphocytes. Indeed, we found that the reported inhibition of TCR-induced Ca2+ influx by M beta CD treatment is mainly due to (a) nonspecific depletion of intracellular Ca2+ stores and (b) plasma membrane depolarization of T cells. When these side-effects are taken into account, raft disorganization does not alter TCR-dependent Ca2+ signaling. In line with these results, also TCR-induced tyrosine phosphorylation is not inhibited by dispersion of lipid rafts. By contrast, in the same conditions, Ca2+ signaling via the glycosylphosphatidylinositol (GPI)-anchored protein CD59 is totally abolished. These results indicate that, while signaling through GPI-anchored proteins requires lipid raft integrity, CD3-dependent TCR activation occurs independently of cholesterol extraction.en_US
dc.language.isoenen_US
dc.relationNoneen_US
dc.relation.ispartofEUROPEAN JOURNAL OF IMMUNOLOGYen_US
dc.titleLipid rafts and T cell receptor signaling: a critical re-evaluationen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/1521-4141(200211)32:11<3082en_US
dc.identifier.pmid12385028en_US
dc.identifier.scopus2-s2.0-0036857767en_US
dc.identifier.isiWOS:000179276100007en_US
dc.relation.volume32en_US
dc.description.firstpage3082en_US
dc.description.lastpage3091en_US
dc.description.thirdmissionNot applicableen_US
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.orcid0000-0003-0598-6449-
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