Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4687
Title: Lipid rafts and T cell receptor signaling: a critical re-evaluation
Authors: Pizzo, P.
Giurisato, Emanuele 
Tassi, M.
Benedetti, A.
Pozzan, T.
Viola, A.
Issue Date: 2002
Project: None 
Journal: EUROPEAN JOURNAL OF IMMUNOLOGY
Abstract: 
The current model suggesting that raft integrity is required for T cell activation is mostly (but not exclusively) based on the use of drugs, such as methyl-beta-cyclodextrin (M beta CD), that disorganize rafts and inhibit T cell receptor (TCR)-induced Ca2+ influx. Here we show that conditions that disrupt lipid raft integrity do not inhibit TCR triggering in Jurkat cells and normal T lymphocytes. Indeed, we found that the reported inhibition of TCR-induced Ca2+ influx by M beta CD treatment is mainly due to (a) nonspecific depletion of intracellular Ca2+ stores and (b) plasma membrane depolarization of T cells. When these side-effects are taken into account, raft disorganization does not alter TCR-dependent Ca2+ signaling. In line with these results, also TCR-induced tyrosine phosphorylation is not inhibited by dispersion of lipid rafts. By contrast, in the same conditions, Ca2+ signaling via the glycosylphosphatidylinositol (GPI)-anchored protein CD59 is totally abolished. These results indicate that, while signaling through GPI-anchored proteins requires lipid raft integrity, CD3-dependent TCR activation occurs independently of cholesterol extraction.
Description: 
37616
URI: http://hdl.handle.net/20.500.12779/4687
ISSN: 0014-2980
DOI: 10.1002/1521-4141(200211)32:11<3082
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