Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4572
Title: Lectin and lectin-related proteins in lima bean (Phaseolus lunatus L.) seeds: biochemical and evolutionary studies
Authors: Sparvoli, F.
Lanave, C.
Santucci, Annalisa 
Bollini, R.
Lioi, L.
Keywords: ALPHA-AMYLASE INHIBITOR; GENETIC DIVERSITY; VULGARIS L; FABACEAE; ARCELIN; PHYTOHEMAGGLUTININ; FAMILY; FRAGMENTATION; RESISTANT; MARKERS
Issue Date: 2001
Project: None 
Journal: PLANT MOLECULAR BIOLOGY
Abstract: 
Lectin-related polypeptides are a class of defence proteins found in seeds of Phaseolus species. In Lima bean (P. lunatus), these proteins and their genes have been well characterized in the Andean morphotype, which represents one of the two gene pools of this species. To study the molecular evolution of the lectin family in Lima bean we characterized the polypeptides belonging to this multigene family and cloned the genes belonging to the Mesoamerican gene pool. The latter gene pool contains components similar to those of the Andean pool, namely: an amylase inhibitor-like (AIL), an arcelin-like (ARL) lectin and the less abundant Lima bean lectin (LBL). These proteins originate from an ancestor gene of the lectin type which duplicated to yield the lectin gene and the progenitor of ARL and AIL. In this species, ARL represents an evolutionary intermediate form that precedes AIL. Phylogenetic analysis supports an Andean origin for Lima bean. The molecular evolutionary studies were extended to the genes of common bean and demonstrated that true lectin genes and the ancestor of lectin-related genes are the result of a duplication event that occurred before speciation. Lima and common bean followed different evolutionary pathways and in the latter species a second duplication event occurred that gave rise, in Mesoamerican wild genotypes, to arcelin genes.
Description: 
37426
URI: http://hdl.handle.net/20.500.12779/4572
ISSN: 0167-4412
DOI: 10.1023/A:1010647310311
Appears in Collections:Publications

Show full item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.