Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4469
Title: Immobilisation of sulphatede hyaluronan for improved biocompatibility
Authors: Barbucci, Rolando
Magnani, Agnese 
R., Rappuoli
Lamponi, Stefania 
M., Consumi
Keywords: Sulphated hyaluronan; Hydrogels; Photoimmobilisation process; Cell mobilisation
Issue Date: 2000
Project: None 
Journal: JOURNAL OF INORGANIC BIOCHEMISTRY
Abstract: 
Hyaluronan (Hyal) was modified by the insertion of sulphate to hydroxyl groups. A series of heparin-like compounds with controllable properties was obtained. The physicochemical and biological behaviours of all these sulphated hyaluronan acids (HyalSx) and their complexes with heavy metal ions (Cu2+ and Zn2+) were investigated. HyalS, derivatives showed a good anticoagulant activity and low platelet aggregation which increased with increasing degree of sulphation. Moreover HyalSx and their Cu2+ complexes were demonstrated to favour the growth of human endothelial cells. However, the utilisation of HyalSx as a material is hindered by its high solubility in physiological solution. Our approach to improve its stability was directed to the synthesis of new HyalSx-based hydrogels and on the preparation of new biocompatible polymeric surfaces obtained through covalent photoimmobilisation of HyalSx. The reaction of primary ovine chondrocytes and B10D2 endothelial cells was studied on both matrices in terms of cell number, F-actin and CD44 receptor immunostaining. Analysis of cell movement showed that the cells respond to HyalSx showing good adhesion and spreading. These results suggest that HyalSx containing materials could be used as biomaterials to aid cartilage repair and vessel endothelisation.
Description: 
37709
URI: http://hdl.handle.net/20.500.12779/4469
ISSN: 0162-0134
Appears in Collections:Publications

Show full item record

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.