Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4418
Title: Effects of surfactants on the spectral behaviour of calcein (II). A method of evaluation
Authors: Memoli, A.
Palermiti, L. G.
Travagli, Valter 
Alhaique, F.
Issue Date: 1999
Project: None 
Journal: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Abstract: 
The spectral behavior of calcein, a water-soluble self quenching fluorescent marker often used in biomedical analysis, can be considerably affected by the presence of surfactants. With this study we intend to obtain further information on the photophysical properties of calcein, in the presence of surfactants and in the concentration range commonly used to investigate the release of such marker from vesicle dispersions. The experiments were carried out both in water and in a physiological buffer (HEPES, pH 7.5), in the presence of Triton X-100, sodium dodecyl sulphate and centyltrimethylammonium bromide, both below and above their critical micelle concentration (c.m.c.). The obtained results confirm that calcein fluorescence can be affected by the presence of surfactants. Thus, environmental conditions must always be carefully checked for the actual quantitative evaluation of this dye. Furthermore, this study sheds some light on the nature and mechanism of calcein quenching.
Description: 
44696
URI: http://hdl.handle.net/20.500.12779/4418
ISSN: 0731-7085
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