Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4414
Title: The role of plasma proteins and stress in the assesment of hemocompatibility
Authors: Barbucci, Rolando
Lamponi, Stefania 
Aloisi, ANNA MARIA
Keywords: platelets, proteins, fibrinogen, stress condition, polyethylene
Issue Date: 1999
Project: None 
Journal: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
Abstract: 
The physiological and psychological conditions of subjects supplying blood for hemocompatibility tests significantly affect the behavior of platelets in terms of both adhesion and activation. The responses of platelets to a standard biomaterial, polyethylene (PE), were examined with blood collected from male rabbits both in basal conditions and after stress, Different media were utilized. First, platelet-rich plasma (PRP) was used to obtain a PE response to contact with platelets. Then platelets drawn from PRP were isolated and washed with Krebs-Ringer solution. One aliquot was suspended in serum (Pw-S) where fibrinogen was absent, another aliquot in Krebs-Ringer solution (Pw-KR) tin order to avoid the influence of the plasma proteins on platelets), and a third aliquot in the original plasma from which the platelets were drawn (Pw-PPP) tin order to restore the initial condition of the plasma but with washed platelets). The analysis of platelet adhesion and morphology was performed by Scanning Electron Microscopy (SEM). Differences in platelet adhesion and morphology were observed with four different media in nonstressed animals, with Pw-PPP showing a higher number and Pw-S and PW-KR lower numbers. Platelet morphology indicated low levels of activation. The platelets drawn from stressed subjects could not be counted in either PRP or PPP medium because they were fully aggregated and adhered; in contrast, in Pw-KR and Pw-S, no significant differences were found with respect to nonstressed conditions, and there was little difference in platelet morphology. All of these factors underline the role of plasma proteins, in particular fibrinogen, in the stress-induced activation of platelets
Description: 
19935
URI: http://hdl.handle.net/20.500.12779/4414
ISSN: 0021-9304
DOI: 10.1002/(SICI)1097-4636(199908)46:2<186
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