Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4391
Title: A sialoglycoprotein, gp20, of the human capacitated sperm surface is a homologue of the leukocyte CD52: analysis of the effect of anti-CD52 monoclonal antibody (CAMPATH-1) on capacitated spermatozoa
Authors: Focarelli, Riccardo
Francavilla, S.
Francavilla, F.
DELLA GIOVAMPAOLA, Cinzia
Santucci, Annalisa 
Rosati, Floriana
Keywords: capacitation; CD52; glycoproteins; leukocytes; spermatozoa
Issue Date: 1999
Project: None 
Journal: MOLECULAR HUMAN REPRODUCTION
Abstract: 
In this study we performed N-terminal sequence analysis of gp20, a 20 kDa sialoglycoprotein on the human sperm surface previously identified by radiolabelling of the sialic acid residues of sperm surface. We found 100% identity with the N-terminus of CD52, an antigen expressed on almost all human leukocytes. We also show that, like CD52, gp20 behaves as a glycosylphosphatidylinositol (GPI)-anchored protein and that anti-gp20 antiserum reacts with an antigen on leukocytes of the same molecular weight as CD52. Using CAMPATH-1, the monoclonal antibody against CD52, in fluorescent staining of capacitated spermatozoa, Western blot analysis and the zona-free hamster egg penetration test, we found that the effect of this antibody was different from that of our anti-gp20. Western blot analysis revealed a well-defined 20 kDa band with anti-gp20, whereas a 14–20 kDa band was detected with CAMPATH-1. Anti-gp20 stained the equatorial region of the sperm head, whereas CAMPATH-1 stained the tail in immunofluorescence analysis of capacitated spermatozoa. A dose-dependent inhibitory effect was seen with CAMPATH-1, similar to that previously detected with anti-gp20, in a zona-free hamster egg penetration test. However, with CAMPATH-1 agglutination of motile spermatozoa was detected, and this was not present with anti-gp20. This suggests that the epitopes recognized by the two antibodies are different.
Description: 
37687
URI: http://hdl.handle.net/20.500.12779/4391
ISSN: 1360-9947
DOI: 10.1093/molehr/5.1.46
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