Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4298
DC FieldValueLanguage
dc.contributor.authorOrlandini, Maurizioen_us
dc.contributor.authorSemplici, F.en_us
dc.contributor.authorFerruzzi, R.en_us
dc.contributor.authorMeggio, F.en_us
dc.contributor.authorPinna, L. A. .en_us
dc.contributor.authorOliviero, Salvatoreen_us
dc.date.accessioned2021-03-30T14:43:04Z-
dc.date.available2021-03-30T14:43:04Z-
dc.date.issued1998-
dc.identifier.issn0021-9258en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12779/4298-
dc.description38358en_US
dc.description.abstractProtein kinase CK2 is an ubiquitous and pleiotropic Ser/Thr protein kinase composed of two catalytic (α and/or α′) and two noncatalytic (β) subunits forming a heterotetrameric holoenzyme involved in cell growth and differentiation. Here we report the identification, cloning, and oncogenic activity of the murine CK2α′ subunit. Serum treatment of quiescent mouse fibroblasts induces CK2α′ mRNA expression, which peaks at 4 h. The kinetics ofCK2α′ expression correlate with increased kinase activity toward a specific CK2 holoenzyme peptide substrate. The ectopic expression of CK2α′ (or CK2α) cooperates with Ha-ras in foci formation of rat primary embryo fibroblasts. Moreover, we observed that BALB/c 3T3 fibroblasts transformed with Ha-ras and CK2α′ show a faster growth rate than cells transformed with Ha-rasalone. In these cells the higher growth rate correlates with an increase in calmodulin phosphorylation, a protein substrate specifically affected by isolated CK2 catalytic subunits but not by CK2 holoenzyme, suggesting that unbalanced expression of a CK2 catalytic subunit synergizes with Ha-ras in cell transformation.en_US
dc.language.isoenen_US
dc.relationNoneen_US
dc.relation.ispartofTHE JOURNAL OF BIOLOGICAL CHEMISTRYen_US
dc.titleProtein kinase CK2a' is induced by serum as a delayed early gene and cooperates with Ha-ras in fibroblast transformation.en_US
dc.typeArticleen_US
dc.identifier.doi10.1074/jbc.273.33.21291en_US
dc.identifier.pmid9694889en_US
dc.identifier.scopus2-s2.0-0032516522en_US
dc.identifier.isiWOS:000075386100082en_US
dc.identifier.urlhttp://www.jbc.org/content/273/33/21291.longen_US
dc.relation.volume273en_US
dc.description.firstpage21291en_US
dc.description.lastpage21297en_US
dc.description.thirdmissionNot applicableen_US
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.orcid0000-0002-6112-4889-
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