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Title: A two-dimensional protein map of human amniotic fluid at 17 weeks' gestation
Authors: Liberatori, S.
Bini, Luca
Magi, Barbara
Marzocchi, Barbara 
Raggiaschi, R.
Frutiger, S.
Sanchez, J. C.
Wilkins, M. R.
Hughes, G.
Hochstrasser, D. F.
Bracci, R.
Pallini, Vitaliano
Issue Date: 1997
Project: None 
Using updated technical procedures (immobilized pH gradients for isoelectric focusing followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: IPG/SDS-PAGE) we provide a two-dimensional (2-D) map of amniotic fluid (AF) proteins. This map comprises over 800 silver-stained spots. Over 150 spots have been identified by matching on the net with human plasma and cerebrospinal fluid maps available from SWISS 2DPAGE database; several additional spots were assigned by immunoblotting and/or microanalytical techniques. This report details our investigation on AF proteins focusing on the 17th week of gestation, when AF is most commonly used for clinical evaluation of fetal disorders. As a whole, the map displays a number of potential markers for fetal development and for gestation abnormalities. The 2-D electrophoretic technique allows the monitoring of all these proteins at the same time along with additional spots that may prove of diagnostic significance.
ISSN: 0173-0835
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