Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4096
Title: Iron release, membrane-protein oxidation and erythrocyte aging
Authors: Signorini, Cinzia
Ferrali, M.
Ciccoli, Lucia
Sugherini, L.
Magnani, Agnese 
Comporti, M.
Issue Date: 1995
Project: None 
Journal: FEBS LETTERS
Abstract: 
The aerobic incubation of erythroeytes in phosphate buffer for 24-60 h (a model of rapid in vitro ageing) induced progressive iron release and methemoglobin formation. Membrane proteins showed electrophoretic alterations and increase in carbonyl groups (as documented by IR spectroscopy). None of these phenomena were seen when the erythrocytes were incubated under anaerobic conditions. The membranes from aerobically incubated cells bound a much higher amount of autologous IgG than those from anaerobically incubated ones, suggesting that the aerobic incubation gives rise to the senescent antigen. The addition of ferrozine during the aerobic incubation prevented both the IgG binding and the protein alterations seen in the IR spectra, suggesting an intracellular chelation of the released iron by ferrozine
Description: 
55624
URI: http://hdl.handle.net/20.500.12779/4096
ISSN: 0014-5793
Appears in Collections:Publications

Show full item record

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.