Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12779/4035
DC FieldValueLanguage
dc.contributor.authorD. Y., Landoen_us
dc.contributor.authorS. G., Haroutiunianen_us
dc.contributor.authorA. M., Kulbaen_us
dc.contributor.authorE. B., Dalianen_us
dc.contributor.authorP., Oriolien_us
dc.contributor.authorMangani, Stefanoen_us
dc.contributor.authorA. A., Akhremen_us
dc.date.accessioned2021-03-30T14:41:16Z-
dc.date.available2021-03-30T14:41:16Z-
dc.date.issued1994-
dc.identifier.issn0739-1102en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12779/4035-
dc.description47981en_US
dc.description.abstractThe theoretical approach to the calculation of the influence of selective binding of small ligands on DNA helix-coil transition has been described in the previous paper (Lando D.Yu., J. Biomol. Struct. Dyn., (1994)). In the present paper that method is used for the study of DNA protonation and deprotonation in acidic and alkaline medium by theoretical analysis of pH effect on DNA heat denaturation. The mechanism of DNA protonation in acidic medium and pk values of nucleotides are well known. It gave us an opportunity to check the theory without any fitting of pK values. A good agreement between experimental and calculated functions T-m(pK) and Delta T(pH) (melting temperature and melting range width) obtained for acidic medium proved the validity of the theory. However, for alkaline medium there was not even qualitative agreement when the agreed-upon mechanism of deprotonation was considered. Looking into the cause of the discrepancy, we have studied the DNA melting for different mechanisms of deprotonation by calculation of T-m(pH) and Delta T(pH). As a result, it has been established that the discrepancy is due to deprotonation of bonded GC base pairs of helical DNA regions (pK=11). It was shown that the early known protonation and newly found deprotonation of helical DNA essentially stabilised double helix in alkaline and acidic medium.en_US
dc.language.isoenen_US
dc.relationNoneen_US
dc.relation.ispartofJOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICSen_US
dc.subjectDNA; ligand; calorimetryen_US
dc.titleTheoretical and Experimental-study of Dna Helix-coil Transition In Acidic and Alkaline-mediumen_US
dc.typeArticleen_US
dc.identifier.doi10.1080/07391102.1994.10508745en_US
dc.identifier.pmid7702774en_US
dc.identifier.scopus2-s2.0-0028138549en_US
dc.identifier.isiWOS:A1994PW09900006en_US
dc.relation.volume12en_US
dc.relation.issue2en_US
dc.description.firstpage355en_US
dc.description.lastpage366en_US
dc.description.thirdmissionNot applicableen_US
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeArticle-
item.fulltextNo Fulltext-
crisitem.author.orcid0000-0003-4824-7478-
Appears in Collections:Publications
Show simple item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.